meeting 27 - 20 october

time: 4.30pm - 6pm
all present

Suggestions of failure:
- wrong experimentation design
- extracting failure
- did not use ginger (pure) immediately
- combination would have cause a better effect

Lime: results shown

Why lime gave the results?
- Properties (Physical/Chemical)

Does lime acidity (pH) has any effect on the zone of inhibition?
If yes, how to prove?

- What causes the inhibition in lime? The active ingredients?

- No consistency in the results. Need to state how much is the different. Double?

* Need to tabulate results!

What cause the different results in the runs?
- storage of extract
- amount
- different people measure (not valid!)
- time of measurement

- Why S.epidymidis? Why use e.coli?

Things need to be done:

- Send Aliza TLC protocol
- Next lab session: Monday, 26th
- Report

1. result of run 3:

Lpg - 1.1cm
nab - 0.9cm


nab - 0.8cm
L - 1.1cm


nab - 2cm
Lpg - 1.5cm


nab - 2.2cm
L - 1.6cm
Leg - 1.1cm

2. summary of all 3 runs:


3. reasons for inconsistency in the result:

meeting 26 - 16 october

time: waiting for someone to tell me
present: waiting for someone to tell me

s.epi serial dilution plates result
did anyone count the cfu???

meeting 25 - 15 october

10.30 – 11. 15 am (aina and fadh)
4.30- 5.30 pm (aina and fadh)

Check 2nd S.epi OD from 6pm (yesterday) to 10.45am (today). (BS) --- 1.625

Do inoculation for 3rd S.epi (CS) from a S.epi streak plate (100ul in 10ml broth) at 11am and check OD at 4.45pm. --- 0.108

Do serial dilution from 3rd S.epi. and do spread plate and incubate at 5.30pm.