meeting 13 - 10 september

Time: 8.30 to 5.30 pm

1) Check OD for the different concentration of pure juice and hot water

Pure juice

A- 5ml broth & 50ul e.coli & 200 ul extract ------------- 2.0049
B-5ml broth & 50ul e.coli & 300 ul extract ------------- 2.0873
C- 5ml broth & 50ul e.coli & 400 ul extract ------------- 2.3452

Hot water

1-5ml broth & 50ul e.coli & 200 ul extract ------------- 1.864
2-5ml broth & 50ul e.coli & 300 ul extract ------------- 1.668
3-5ml broth & 50ul e.coli & 400 ul extract ------------- 2.322

control

5ml broth & 50 ul e.coli ------------------ 1.868

G --------------- 1.994

2) make LB agar

12.5 g LB + 7.5g agar --> 500 ml water ( 20 plates )
25g LB + 15g agar --> 1000 ml water ( 40 plates )


3) serial dilution for the pure juice, hot water and control

- measure 900 ul of saline into 100 ul of bacteria into the A, B, C, 1, 2,3 and Control. eg:

A

10^1
10^2
10^3
10^4
10^5
10^6
10^7

from there, only 10^4 until 10^7 for each set were plated and incubate at 5.30 pm