Rules and regulation

1. This project officially ended on 12 December 2009.

2. If you posted anything or edited anything, please credit yourself as the author.

3. Please just favorite this blog for teammates.

4. Do not link in blog to keep in confidential to other FYP team. (Now you have the right to link it to your blog. :)

5. For outsiders visiting this blog, please do not copy any of our contents or photographs without all our teammates permission. Offenders will be reported.

6. Lastly, please practice PROFESSIONALISM.

7. To copy logbook:
meeting 1 - 13 august

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Wednesday, September 9, 2009

meeting 11 - 8 september

Time: 8.30am - 4pm

1. check od
time: 3.18pm - 8.50am
od: 1.943

2. rotary evaporator
hot water: 72mbar, vapor temp - 41dc, 80rpm, water bath - 95dc
ethanol: 175mbar, vapor temp - 42dc, 70rpm, water bath - 65dc
chiller: 5dc

3. hot water extract
extract with flask - 93.440g
extract diluted with 1ml distilled water
empty flask - 93.108g
extract - 0.332g
concentration - 0.332g/ml

4. serial dilution of hot water extract
100ul into 900ul of distilled water
do till 10^5 dilution

5. disk and wel diffusion method
disk: 30ul, well: 50ul
well - 3.48pm - 9.15am
disk - 3.56pm - 9.15am

6. innoculation
time: 3pm - 9.15am