1. check od
time: 3.18pm - 8.50am
od: 1.943
2. rotary evaporator
hot water: 72mbar, vapor temp - 41dc, 80rpm, water bath - 95dc
ethanol: 175mbar, vapor temp - 42dc, 70rpm, water bath - 65dc
chiller: 5dc
3. hot water extract
extract with flask - 93.440g
extract diluted with 1ml distilled water
empty flask - 93.108g
extract - 0.332g
concentration - 0.332g/ml
4. serial dilution of hot water extract
100ul into 900ul of distilled water
do till 10^5 dilution
5. disk and wel diffusion method
disk: 30ul, well: 50ul
time:
well - 3.48pm - 9.15am
disk - 3.56pm - 9.15am
6. innoculation
time: 3pm - 9.15am
