PLEASE NOTE THAT NOT EVERYTHING WRITTEN HERE IS IN DETAILED. ADD IN YOUR OWN NOTES AND REASONING IN YOUR OWN LOG BOOK.
Time: 9.30am - 3.30pm
Objectives: Fill in yourself
- Making of agar plates
25g LB + 15g agar into 1000ml
- Broth
18.75g LB into 750ml
- Saline
2.25g NaCl into 250ml
- Sodium benzoate
7.5g powder into 15ml
Negative control changed to sterilized distilled water.
- Might have plan to remove Lemon grass (Not confirmed yet)
Thinking of replacing with Pandan leaves. (Not confirmed yet)
- Extraction of Ginger
Things needed:
Muslin cloth/ Cheese cloth
90% or 95% Ethanol
- Innoculation
Put 100 ul of e.coli in 10ml of broth in a conical flask and incubate it at 37 degree celcius at 100 RPM. (2.40pm)
- Next plan of action
26 Aug Wed - innoculation
Thurs - discuss further on extraction method
- check OD
- streak plate
- is there a need to innoculate (maintain consistency)
Fri - check OD
- discuss further on extraction method
Next week
Mon - take one colony from streak plate and innoculate
Tues - serial dilution
Wed - count CFU
Posted by: Pei Yi and Aina
Rules and regulation
1. This project officially ended on 12 December 2009.
2. If you posted anything or edited anything, please credit yourself as the author.
3. Please just favorite this blog for teammates.
4.Do not link in blog to keep in confidential to other FYP team. (Now you have the right to link it to your blog. :)
5. For outsiders visiting this blog, please do not copy any of our contents or photographs without all our teammates permission. Offenders will be reported.
6. Lastly, please practice PROFESSIONALISM.
7. To copy logbook:
meeting 1 - 13 august
2. If you posted anything or edited anything, please credit yourself as the author.
3. Please just favorite this blog for teammates.
4.
5. For outsiders visiting this blog, please do not copy any of our contents or photographs without all our teammates permission. Offenders will be reported.
6. Lastly, please practice PROFESSIONALISM.
7. To copy logbook:
meeting 1 - 13 august
