1) check OD
time: 3pm - 9.15am
od: 1.958
2) check plates
- no zone of inhibition for all the plates except for the NaB.
DISK DIFFUSION
controls, original, 10^1, 10^2
controls, 10^3, 10^4, 10^5
controls, ethanol, pure juice
WELL DIFFUSION
controls, original, 10^1, 10^2
controls, 10^3, 10^4, 10^5
controls, ethanol, pure juice
3) do saline : 250 ml of water with 2.25g of NaCl and autoclave at 12
4) do a bigger paper disk (using the cap of a green falcon tube) and autoclave at 12
5) innoculate different concentration of pure juice and hot water.
Pure juice
A- 5ml broth & 50ul e.coli & 200 ul extract
B-5ml broth & 50ul e.coli & 300 ul extract
C- 5ml broth & 50ul e.coli & 400 ul extract
Hot water
1-5ml broth & 50ul e.coli & 200 ul extract
2-5ml broth & 50ul e.coli & 300 ul extract
3-5ml broth & 50ul e.coli & 400 ul extract
control
5ml broth & 50 ul e.coli
6) innoculate e.coli, 100ul of e.coli in 10 ml of broth (G)
7) do a set of all the controls using well diffusion method
- NaB
- Ethanol
- Water
- Saline
**** all the innoculation and controls were incubate at 2.30 pm
