Rules and regulation

1. This project officially ended on 12 December 2009.

2. If you posted anything or edited anything, please credit yourself as the author.

3. Please just favorite this blog for teammates.

4. Do not link in blog to keep in confidential to other FYP team. (Now you have the right to link it to your blog. :)

5. For outsiders visiting this blog, please do not copy any of our contents or photographs without all our teammates permission. Offenders will be reported.

6. Lastly, please practice PROFESSIONALISM.

7. To copy logbook:
meeting 1 - 13 august

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Wednesday, September 9, 2009

meeting 12 - 9 septemebr

Time: 9am-3 pm

1) check OD
time: 3pm - 9.15am
od: 1.958

2) check plates
- no zone of inhibition for all the plates except for the NaB.

DISK DIFFUSION

controls, original, 10^1, 10^2

controls, 10^3, 10^4, 10^5
controls, ethanol, pure juice

WELL DIFFUSION

controls, original, 10^1, 10^2

controls, 10^3, 10^4, 10^5

controls, ethanol, pure juice



3) do saline : 250 ml of water with 2.25g of NaCl and autoclave at 12

4) do a bigger paper disk (using the cap of a green falcon tube) and autoclave at 12

5) innoculate different concentration of pure juice and hot water.

Pure juice

A- 5ml broth & 50ul e.coli & 200 ul extract
B-5ml broth & 50ul e.coli & 300 ul extract
C- 5ml broth & 50ul e.coli & 400 ul extract

Hot water

1-5ml broth & 50ul e.coli & 200 ul extract
2-5ml broth & 50ul e.coli & 300 ul extract
3-5ml broth & 50ul e.coli & 400 ul extract

control

5ml broth & 50 ul e.coli

6) innoculate e.coli, 100ul of e.coli in 10 ml of broth (G)

7) do a set of all the controls using well diffusion method


- NaB
- Ethanol
- Water
- Saline

**** all the innoculation and controls were incubate at 2.30 pm