What do we gather?
1) Do LB agar
--> 2 500ml bottles
--> 12.5g LB + 7.5g agar = 500ml distilled water
--> total made: 1000ml
2) Inoculate bacteria
3) Put ethanol extract in vacuum concentrator.
--> We decided not to put it in the rotary evaporator.
--> Aliza suggest we put it in the vacuum concentrator to spin dry the extract. (we got use before last sem)
--> separate the extract into 2 microtubes.
--> put in vacuum concentrator overnight at 60dc. (3.30pm-10am)
--> empty microtubes A- 1.0554g & B- 1.0550g
Next plant of action:
- check OD
- pour plate
- do agar well and disk diffusion
- check ethanol in the vacuum concentrator