1. Check OD
time: 4pm - 9.30am
e.coli = 1.508
2. growth curve
using one colony from streak plate and put into broth.
incubate at 10.50am, 37dc and 100rpm.
measure every 1 hour +/- to obtain 6 points to plot the graph.
11.50am - 0
1ml was mistakenly thrown away by lala
1.18pm - 0.183
2.18pm - 0.669
3.18pm - 1.378
3.30pm - 1.438
4pm - 1.656
[draw the graph yourself.]
3. preparation of ginger
washed > peeled > washed > dried > cut > blended > 1,2,3
1 - hot water
- 200g soaked in 100ml of hot water for 24 hours (1.30pm - 1.30pm next day)
- resultant juice extracted, stored in bottle and placed in box and ready to use for testing.
2 - 95% ethanol
- 200g soaked in 100ml of ethanol for 24 hours (1.30pm - 1.30pm next day)
- extract obtained, stored in bottle and placed in box for further processing.
3 - cloth
- 200g.
- pulp were separated using cloth
- pure juice was extracted and stored in dark bottle at 4dc.
4. serial dilution
time: 4pm - 10.19am
5. innoculation
time: 4pm - 10.19am
