(aina, fadh, shila):
arfa left early:
py absent
1. make trolox solution
0.0015g into 50ul h20
2. label the plate
+, -, L, G
3. put ?ul of each solvent onto TLC plate
4. pour toluene and ethyl ecetate into a beaker and cover with aluminium foil. fill until half of the pencil marking on the tlc plate.
5. run the plate until almost near the top. draw a line.
6. air dry the plate
7. immerse in 5% ethanolic sulphuric acid and then 1% ethanolic vanillin.
8. heat the tlc plate on hotplate until color spot appear.
Result:
please interpret together.
